Difference between revisions of "Part:BBa K4364001:Experience"
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We used this part in combination with BBa_K608003 to create an expression construct that produces VIM-2 MBL. We proved that the resulting construct was functional and we used the secreted MBL enzyme to treat ampicillin contaminations in water samples (see https://2023.igem.wiki/bulgaria/engineering). | We used this part in combination with BBa_K608003 to create an expression construct that produces VIM-2 MBL. We proved that the resulting construct was functional and we used the secreted MBL enzyme to treat ampicillin contaminations in water samples (see https://2023.igem.wiki/bulgaria/engineering). | ||
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Ampicillin degradation was observed but the NDM-2&5-based version was several times more efficient and we recommend its usage instead of this part. | Ampicillin degradation was observed but the NDM-2&5-based version was several times more efficient and we recommend its usage instead of this part. | ||
Revision as of 00:16, 12 October 2023
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4364001
This part was used to generate constructs with constitutive expression that were next used for the CADABRA cocktail. See our Wiki for more details. [http://2022.igem.org/Team:Bulgaria]
User Reviews
UNIQ88b53ed6966c8475-partinfo-00000000-QINU UNIQ88b53ed6966c8475-partinfo-00000001-QINU
User review of iGEM Bulgaria 2023
We used this part in combination with BBa_K608003 to create an expression construct that produces VIM-2 MBL. We proved that the resulting construct was functional and we used the secreted MBL enzyme to treat ampicillin contaminations in water samples (see https://2023.igem.wiki/bulgaria/engineering).
File:Https://static.igem.wiki/teams/4855/wiki/amp-test.png
Ampicillin degradation was observed but the NDM-2&5-based version was several times more efficient and we recommend its usage instead of this part.