Difference between revisions of "Part:BBa K4604006"
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| − | This BioBrick is a mutated Version of BioBrick BBa_K4604005, the gene for the | + | <html> |
| + | This BioBrick is a mutated Version of BioBrick <a href="https://parts.igem.org/Part:BBa_K4604005">BBa_K4604005</a>, the gene for the BluB enzyme that catalyzes the reaction of FMNH2 to DMB used in the AdoCbl synthesis pathway in <i>E. coli</i>. Also used in <a href="https://parts.igem.org/Part:BBa_K4604020">BBa_K4604020</a> , a more detailed characterization is uploaded there. Has a C-terminal his-tag.</html> | ||
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| + | <figure><center> | ||
| + | <img src="https://static.igem.wiki/teams/4604/wiki/description/b12-production.png" width="700"> | ||
| + | </figure></html> | ||
| + | <span style="font-size: smaller;"><b>Figure 1: Vitamin B12 production from precursors in <i>E. coli</i>.</b> The BluB transforms the reduced form of flavin mononucleotide (FMNH2) to form 5,6-dimethylbenzimidazole (DMB) which then joins exogenously-provided cobinamide to create vitamin B12. Made with biorender.com</span> | ||
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Latest revision as of 23:10, 11 October 2023
bluB mutated
This BioBrick is a mutated Version of BioBrick BBa_K4604005, the gene for the BluB enzyme that catalyzes the reaction of FMNH2 to DMB used in the AdoCbl synthesis pathway in E. coli. Also used in BBa_K4604020 , a more detailed characterization is uploaded there. Has a C-terminal his-tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]