Difference between revisions of "Part:BBa K283007:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Reference:Polar localization of the autotransporter family of large bacterial virulence proteins.Journal of Bacteriology, 2006vol.188(no.13) | |
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===Source=== | ===Source=== |
Revision as of 11:18, 20 October 2009
T7 RNA polymerase
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1693
Design Notes
Reference:Polar localization of the autotransporter family of large bacterial virulence proteins.Journal of Bacteriology, 2006vol.188(no.13)
Source
Pathogenic Escherichia coli strain 2787 (O126:H27) The genes encoding AIDA (adhesin involved in diffuse adherence) are located on two open reading frames, orfA and orfB. The latter codes for the AIDA autotransporter system, which is synthesized as a pre-pro-protein that is C-terminally processed to generate the mature adhesin AIDA-I and the outer membrane integrated AIDAC, which functions as the translocator for AIDA-I. orfA codes for a 45-kDa cytoplasmic protein which is required to modify AIDA-I such that it adheres to target cells. Although the AIDA system has first been characterized in a clinical E. coli isolate responsible for infant diarrhea, AIDA genes have been detected only in about 2 to 4% of human isolates . Thus, an animal reservoir for AIDA may exist.