Difference between revisions of "Part:BBa K4757062"

 
Line 5: Line 5:
 
Terephthalic acid sensing transcription factor - fluorescence mKate2 expression cassette.....
 
Terephthalic acid sensing transcription factor - fluorescence mKate2 expression cassette.....
 
to be continued...
 
to be continued...
 +
 +
Engnineering journey
 +
<p>Mutation -> weak expression/no expression after mutation -> enhancing with RBS -> still weak/no expression -> co-induction -> significant changes could be detected </p>
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:37, 11 October 2023


TPA sensing XylS-K38R-L224Q/Pm expressing mKate2

Terephthalic acid sensing transcription factor - fluorescence mKate2 expression cassette..... to be continued...

Engnineering journey

Mutation -> weak expression/no expression after mutation -> enhancing with RBS -> still weak/no expression -> co-induction -> significant changes could be detected


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 212
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 934
  • 1000
    COMPATIBLE WITH RFC[1000]