Difference between revisions of "Part:BBa F1610"

 
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<partinfo>BBa_F1610 short</partinfo>
 
<partinfo>BBa_F1610 short</partinfo>
  
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This device accepts PoPS as input and produces the LuxI enzyme.  This enzyme produces 3OC<sub>6</sub>HSL.
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This device accepts [[PoPS]] as input and produces the LuxI enzyme.  This enzyme produces [[3OC6HSL|3OC<sub>6</sub>HSL]].
  
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===Notice===
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'''With a VF2-VR PCR length compraison, I found that it is empty on this plasmid sent in iGEM2007 plates.
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'''
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--[[User:MaRui|marion]] 07:12, 22 March 2008 (EDT)
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'''While present on a gel after running PCR on both 2007 and 2008 plates, the DNA appears to only be ~600bp long which conflicts with the part description.''' --[[robere]] 27 Jun 2008<br>
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[[:Image:F1610_PCR_gel.jpg|See gel image]]
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'''The part we used is in iGEM2009 plate. After cutting the plasmid in EcoRI and Spel restriction sites, we separated the fragments by electrophoresis. There appeared two bands, one is about 3100bp, and the other is about 800bp.'''--[[User:Koi|Koi]]  20 Oct 2009 (Tokyo_Tech)
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[[:Image:F1610_restriction2009.jpg|See gel image]]
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===Usage and Biology===
 
===Usage and Biology===
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-- Please enter your experience with this part here --
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_F1610 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_F1610 SequenceAndFeatures</partinfo>
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_F1610 parameters</partinfo>
 
<partinfo>BBa_F1610 parameters</partinfo>
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Latest revision as of 10:09, 20 October 2009

3OC6HSL Sender Device

This device accepts PoPS as input and produces the LuxI enzyme. This enzyme produces 3OC6HSL.

Notice

With a VF2-VR PCR length compraison, I found that it is empty on this plasmid sent in iGEM2007 plates. --marion 07:12, 22 March 2008 (EDT)


While present on a gel after running PCR on both 2007 and 2008 plates, the DNA appears to only be ~600bp long which conflicts with the part description. --robere 27 Jun 2008
See gel image


The part we used is in iGEM2009 plate. After cutting the plasmid in EcoRI and Spel restriction sites, we separated the fragments by electrophoresis. There appeared two bands, one is about 3100bp, and the other is about 800bp.--Koi 20 Oct 2009 (Tokyo_Tech)

See gel image

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]