Difference between revisions of "Part:BBa K4855000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part was designed as a gBlock fragment and ordered from IDT. | + | This part was designed as a gBlock fragment and ordered from IDT. C-terminal 6xHis tag was added for purifications. |
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===Source=== | ===Source=== | ||
Revision as of 21:47, 11 October 2023
blaNDM-2&5
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was designed as a gBlock fragment and ordered from IDT. C-terminal 6xHis tag was added for purifications.
Source
This part was based on protein sequences NDM-2 (F2YZ26) and NDM-5 (A0A2S1PIF3). The final sequence was codon-optimized for optimal expression in E. coli.