Difference between revisions of "Part:BBa K4960013"
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Pvc14 possibly controls tube optimal length.
 
===Profile===
 
===Profile===
 
Name: pvc14<br>
 
Name: pvc14<br>

Revision as of 21:41, 11 October 2023


pvc14
Pvc14 possibly controls tube optimal length.

Profile

Name: pvc14
Base Pairs: 1716bp
Origin: Photorhabdus
Properties: Pvc14 possibly controls tube optimal length

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

Pvc14 is a homolog of Afp14 in Serratia Afp, which was proposed to act as a tape measure protein.According to previous studies,it is present at very low levels in the PVC syringes sample.Significantly,its deletion resulted in a large variation in the length of PVC particles.Therefore,it possibly controls tube optimal length.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.