Difference between revisions of "Part:BBa K4830008:Design"

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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K4830008 short</partinfo>
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<partinfo>BBa_K4830009 short</partinfo>
  
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<partinfo>BBa_K4830008 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4830009 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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The design of POAG snp1-3 pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail.
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The design of ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail. The unique ngRNA was designed based on SNP data.
  
 
===Source===
 
===Source===
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The pegRNA design was based on SNP data obtained from collaboration with clinician.
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SNPs were retrieved from the paper cited in References.
  
 
===References===
 
===References===
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Gharahkhani, P. et al. (2021) ‘Genome-wide meta-analysis identifies 127 open-angle glaucoma loci with consistent effect across ancestries’, Nature Communications, 12(1). doi:10.1038/s41467-020-20851-4.

Revision as of 20:53, 11 October 2023


POAG snp1-3 ngRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail. The unique ngRNA was designed based on SNP data.

Source

SNPs were retrieved from the paper cited in References.

References

Gharahkhani, P. et al. (2021) ‘Genome-wide meta-analysis identifies 127 open-angle glaucoma loci with consistent effect across ancestries’, Nature Communications, 12(1). doi:10.1038/s41467-020-20851-4.