Difference between revisions of "Part:BBa K4825017"

 
 
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ATF1 is derived from S.cerevisiae genome. It encodes for the enzyme alcohol acetyltransferase 1 (AATase 1), and catalyses the reaction of acetate esters from acetyl-CoA and alcohols. It is included in our composite part with ARO10 (2-ketoacid decarboxylase) and ADH7 (alcohol dehydrogenase), which are transformed into the genome of S.cerevisiae simultaneously for overexpression to produce isoamyl acetate.
 
ATF1 is derived from S.cerevisiae genome. It encodes for the enzyme alcohol acetyltransferase 1 (AATase 1), and catalyses the reaction of acetate esters from acetyl-CoA and alcohols. It is included in our composite part with ARO10 (2-ketoacid decarboxylase) and ADH7 (alcohol dehydrogenase), which are transformed into the genome of S.cerevisiae simultaneously for overexpression to produce isoamyl acetate.
  
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===Usage and Biology===
 
===Usage and Biology===
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ATF1 is a DNA sequence derived from the genome of S.cerevisiae that encodes for the enzyme alcohol acetyltransferase 1 (AATase-1). It catalyzes the conversion from acetyl-CoA and alcohols to acetate esters. During fermentation, the rates of producing acetate esters demonstrate a correlation to AATase activity.
  
 
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Latest revision as of 18:52, 11 October 2023


ATF1

ATF1 is derived from S.cerevisiae genome. It encodes for the enzyme alcohol acetyltransferase 1 (AATase 1), and catalyses the reaction of acetate esters from acetyl-CoA and alcohols. It is included in our composite part with ARO10 (2-ketoacid decarboxylase) and ADH7 (alcohol dehydrogenase), which are transformed into the genome of S.cerevisiae simultaneously for overexpression to produce isoamyl acetate.


Usage and Biology

ATF1 is a DNA sequence derived from the genome of S.cerevisiae that encodes for the enzyme alcohol acetyltransferase 1 (AATase-1). It catalyzes the conversion from acetyl-CoA and alcohols to acetate esters. During fermentation, the rates of producing acetate esters demonstrate a correlation to AATase activity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 412
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 412
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 412
    Illegal BglII site found at 99
    Illegal BamHI site found at 1426
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 412
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 412
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526
    Illegal SapI.rc site found at 1534