Difference between revisions of "Part:BBa K260009"
Line 5: | Line 5: | ||
This plasmid backbone is available with BioBrick P_F3_zeoR_F3_RFP ([[Part:BBa_K260017|BBa_K260017]]) depicted below. The product of the above-described recombineering reaction [[Part:BBa_K260002|BBa_K260002]]-[[Part:BBa_K260017|BBa_K260017]] is also available. | This plasmid backbone is available with BioBrick P_F3_zeoR_F3_RFP ([[Part:BBa_K260017|BBa_K260017]]) depicted below. The product of the above-described recombineering reaction [[Part:BBa_K260002|BBa_K260002]]-[[Part:BBa_K260017|BBa_K260017]] is also available. | ||
+ | |||
[[Image:pMA-RQ-P_F3_zeoR_F3_RFP.jpg]] | [[Image:pMA-RQ-P_F3_zeoR_F3_RFP.jpg]] | ||
Latest revision as of 06:13, 20 October 2009
pMA-RQ-@TetFlp
This plasmid backbone is based on pMA-RQ from Geneart. It has BioBrick prefix and suffix flanked on both sides by 70 bp of homology to pTetFlp (BBa_K260002) for tranfer of the contained BioBrick part by recombineering, for which the BioBrick needs to be excised with PvuII from the backbone.
This plasmid backbone is available with BioBrick P_F3_zeoR_F3_RFP (BBa_K260017) depicted below. The product of the above-described recombineering reaction BBa_K260002-BBa_K260017 is also available.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 67
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2578
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2584 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2578
Illegal XhoI site found at 61
Illegal XhoI site found at 2572 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2578
Illegal suffix found at 2
Illegal XbaI site found at 67 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2578
Plasmid lacks a suffix.
Illegal XbaI site found at 67
Illegal XbaI site found at 2593
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.