Difference between revisions of "Part:BBa K4825006"

 
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In our project, this part is used to secrete heterologous protein Cry1518-35 out of S.cerevisiae, of which the efficiency is examined by secreting red fluorescence protein (RFP). This part can be applied as an advanced attempt for heterologous expression in S.cerevisiae. "
 
In our project, this part is used to secrete heterologous protein Cry1518-35 out of S.cerevisiae, of which the efficiency is examined by secreting red fluorescence protein (RFP). This part can be applied as an advanced attempt for heterologous expression in S.cerevisiae. "
  
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===Usage and Biology===
 
===Usage and Biology===
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OPT, optimized from the native signal peptide in S.cerevisiae, ScMα, is a 267bp signal peptide located in the N-terminal of targeted proteins. Its role in translocating proteins to the periplasmic space or out of the cell starts with the N-region orienting the secretion by interactions with negatively charged phosphate group of the lipid bilayer of the cells, and ends with the C-region being cleaved by signal peptidase. As a result, the targeted heterologous proteins will be secreted to extracellular space, whereas the signal peptide itself remains in the cytosol.
  
 
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Revision as of 18:35, 11 October 2023


OPT

"OPT is an optimized version of the signal peptide originated in S.cerevisiae, ScMα, that can target heterologous proteins to the extracellular environment of yeast cells.

This part as a signal peptide can enhance the ability of S.cerevisiae in secreting heterologous or recombinant enzymes by determining and trafficing the secretion pathway of target genes, and the modifications in the genetic sequence remarkably increase the secretion level in yeasts, compared to the native ScMα signal peptide.

In our project, this part is used to secrete heterologous protein Cry1518-35 out of S.cerevisiae, of which the efficiency is examined by secreting red fluorescence protein (RFP). This part can be applied as an advanced attempt for heterologous expression in S.cerevisiae. "

Usage and Biology

OPT, optimized from the native signal peptide in S.cerevisiae, ScMα, is a 267bp signal peptide located in the N-terminal of targeted proteins. Its role in translocating proteins to the periplasmic space or out of the cell starts with the N-region orienting the secretion by interactions with negatively charged phosphate group of the lipid bilayer of the cells, and ends with the C-region being cleaved by signal peptidase. As a result, the targeted heterologous proteins will be secreted to extracellular space, whereas the signal peptide itself remains in the cytosol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 268
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 268
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 268
    Illegal XhoI site found at 244
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 268
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]