Difference between revisions of "Part:BBa K4825003"

Latest revision as of 18:26, 11 October 2023

PS

pinene synthase (PtPS) is a heterogenous gene from Pinus taeda that can convert GPP into pinene. PS contains N-terminal transport peptides (1-52 loci amino acid residues) in the native host and is responsible for targeting translation products to plastid, however this function is not required in yeast, and the N-terminal transport peptide might cause erring location of the enzyme, decrease in solubility or wrongful folding of the enzyme and affect the efficiency in yeasts, so the N-terminal of PtPS is truncated to form t48PtPS

Usage and Biology

a heterogenous gene from Pinus taeda that can convert GPP into pinene. PS contains n-terminal transport peptides in the native host and is responsible for targeting translation products to plastid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 223
Illegal BamHI site found at 1060
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 695

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 <partinfo>BBa_K4825003 short</partinfo>
-pinene synthase (PtPS) is a heterogenous gene from Pinus taeda that can convert GPP into pinene. PS contains n-terminal transport peptides in the native host and is responsible for targeting translation products to plastid, however this function is not required in yeast, and the N-terminal transport peptide might cause erring location of the enzyme, decrease in solubility or wrongful folding of the enzyme and affect the efficiency in yeasts, so the N-terminal of PtPS is truncated to form tPtPS
+pinene synthase (PtPS) is a heterogenous gene from Pinus taeda that can convert GPP into pinene. PS contains N-terminal transport peptides (1-52 loci amino acid residues) in the native host and is responsible for targeting translation products to plastid, however this function is not required in yeast, and the N-terminal transport peptide might cause erring location of the enzyme, decrease in solubility or wrongful folding of the enzyme and affect the efficiency in yeasts, so the N-terminal of PtPS is truncated to form t48PtPS
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+a heterogenous gene from Pinus taeda that can convert GPP into pinene. PS contains n-terminal transport peptides in the native host and is responsible for targeting translation products to plastid.
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