Difference between revisions of "Part:BBa K4759053"

Revision as of 18:22, 11 October 2023

petH-RBS2-petF(D21Y)

The P450 enzymes are redox-dependent proteins, through which they source electrons from reducing cofactors to drive their activities. This part is coding for ferredoxin reductase PetH and ferredoxin PetF from the algae (Synechocystis PCC 6803) as the redox chaperones of OleP. We construct the plasmid pRSFDuet-petH-petF(D21Y)-olep to express PetH 、 PetF and Olep in E. coli C43.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1249
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1249
Illegal NotI site found at 1022
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1249
Illegal BglII site found at 1560
Illegal BamHI site found at 1243
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1249
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1249
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4759053 short</partinfo>
-The P450 enzymes are redox-dependent proteins, through which they source electrons from reducing cofactors to drive their activities. This part is coding for ferredoxin reductase PetH (SEQ ID NO.7) and ferredoxin PetF (SEQ ID NO.8) from the algae (Synechocystis PCC 6803) as the redox chaperones of OleP. We construct the plasmid pRSFDuet-petH-petF(D21Y)-olep to express PetH &#12289; PetF and Olep in E. coli C43.
+The P450 enzymes are redox-dependent proteins, through which they source electrons from reducing cofactors to drive their activities. This part is coding for ferredoxin reductase PetH and ferredoxin PetF from the algae (Synechocystis PCC 6803) as the redox chaperones of OleP. We construct the plasmid pRSFDuet-petH-petF(D21Y)-olep to express PetH &#12289; PetF and Olep in E. coli C43.
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