Difference between revisions of "Part:BBa K4716007"
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| − | On the direction for our gene expression, there is an Pasr promoter (BBa_K4716000) which is the pH-responsive promoter native to E.coli, inducing transcription in human duodenum region’s relatively acidic environment (pH 5~6), RBS (BBa_B0034) is the ribosome binding site, CsgA-Perpa-mafp-5 is our terget gene, the mSandy2 is the reporter gene and the double terminator (BBa_B0015) is the terminater we use in this circuit, BioBrick prefix and BioBrick suffix are presented at the beginning and end of the whole | + | On the direction for our gene expression, there is an Pasr promoter (BBa_K4716000) which is the pH-responsive promoter native to E.coli, inducing transcription in human duodenum region’s relatively acidic environment (pH 5~6), RBS (BBa_B0034) is the ribosome binding site, CsgA-Perpa-mafp-5 is our terget gene, the mSandy2 is the reporter gene and the double terminator (BBa_B0015) is the terminater we use in this circuit, BioBrick prefix and BioBrick suffix are presented at the beginning and end of the whole insert gene, respectively. In the opposite direction, the cat promoter controls the CmR gene which is the selective marker, carrying chloramphenicaol resistance gene for our screening object. Followed by Lambda T0 terminator, there is also a replication origin for the whole plasmid. |
| − | insert gene, respectively. In the opposite direction, the cat promoter controls the CmR gene which is the selective marker, carrying chloramphenicaol resistance gene for our screening object. Followed by Lambda T0 terminator, there is also a replication origin for the whole plasmid. | + | |
Revision as of 17:54, 11 October 2023
On the direction for our gene expression, there is an Pasr promoter (BBa_K4716000) which is the pH-responsive promoter native to E.coli, inducing transcription in human duodenum region’s relatively acidic environment (pH 5~6), RBS (BBa_B0034) is the ribosome binding site, CsgA-Perpa-mafp-5 is our terget gene, the mSandy2 is the reporter gene and the double terminator (BBa_B0015) is the terminater we use in this circuit, BioBrick prefix and BioBrick suffix are presented at the beginning and end of the whole insert gene, respectively. In the opposite direction, the cat promoter controls the CmR gene which is the selective marker, carrying chloramphenicaol resistance gene for our screening object. Followed by Lambda T0 terminator, there is also a replication origin for the whole plasmid.