Difference between revisions of "Part:BBa K4830029"

 
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The eGFP_complex premature stop codon is a derivative of the eGFP_simple premature stop codon. Similar to the eGFP_simple premature stop codon, the eGFP_complex premature stop codon can be utilized in a Traffic Light Reporter (TLR) assay.
 
The eGFP_complex premature stop codon is a derivative of the eGFP_simple premature stop codon. Similar to the eGFP_simple premature stop codon, the eGFP_complex premature stop codon can be utilized in a Traffic Light Reporter (TLR) assay.
  
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However, the edit required is complex as it involves the truncation in a particular region from 42bp to 31bp.
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However, the edit required is complex as it involves the truncation in a particular region from 42bp to 31bp. Specific pegRNA and ngRNA targeting the premature stop codon have been designed - TLR-76 pegRNA and 777BFP ngRNA.
  
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===Usage and Biology===
 
===Usage and Biology===
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The eGFP_complex premature stop codon is a coding sequence used in the Traffic Light Reporter (TLR) assay. It contains a particular region that requires a complex edit to truncate from 42bp to 31bp, before restoring the wild-type eGFP.
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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The coding sequence spans the eGFP with an inherent premature stop codon to be edited.
 
<partinfo>BBa_K4830029 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4830029 SequenceAndFeatures</partinfo>
  

Revision as of 17:53, 11 October 2023


eGFP_complex premature stop codon

The eGFP_complex premature stop codon is a derivative of the eGFP_simple premature stop codon. Similar to the eGFP_simple premature stop codon, the eGFP_complex premature stop codon can be utilized in a Traffic Light Reporter (TLR) assay.

However, the edit required is complex as it involves the truncation in a particular region from 42bp to 31bp. Specific pegRNA and ngRNA targeting the premature stop codon have been designed - TLR-76 pegRNA and 777BFP ngRNA.

Usage and Biology

The eGFP_complex premature stop codon is a coding sequence used in the Traffic Light Reporter (TLR) assay. It contains a particular region that requires a complex edit to truncate from 42bp to 31bp, before restoring the wild-type eGFP.

Sequence and Features

The coding sequence spans the eGFP with an inherent premature stop codon to be edited.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
    Illegal NgoMIV site found at 734
  • 1000
    COMPATIBLE WITH RFC[1000]