Difference between revisions of "Part:BBa K4830028"

 
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<partinfo>BBa_K4830028 short</partinfo>
 
<partinfo>BBa_K4830028 short</partinfo>
  
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eGFP_premature stop codon is a protein coding sequence that contains the eGFP with a premature stop codon. This coding sequence is particularly useful for Traffic Light Reporter (TLR) assays that screen for DNA editing activity. This sequence is used in a TLR assay for prime editing where pegRNAs and ngRNAs targeting the premature stop codon were designed to swap the premature stop codon into a functional amino acid, restoring the wild-type eGFP.
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eGFP_premature stop codon is a protein coding sequence that contains the eGFP with a premature stop codon. This coding sequence is particularly useful for Traffic Light Reporter (TLR) assays that screen for DNA editing activity.  
  
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When cloned into a suitable plasmid and subsequently transfected into mammalian cells for in vitro DNA editing, successfully edited cells will fluorescence green which can then be analysed with flow cytometry.
 
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===Usage and Biology===
 
===Usage and Biology===
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This sequence is used in a TLR assay for prime editing where pegRNAs and ngRNAs targeting the premature stop codon were designed to swap the premature stop codon into a functional amino acid, restoring the wild-type eGFP.
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When cloned into a suitable plasmid and subsequently transfected into mammalian cells for in vitro DNA editing, successfully edited cells will fluorescence green which can then be analysed with flow cytometry.
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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The sequence contains the eGFP with a premature stop codon.
 
<partinfo>BBa_K4830028 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4830028 SequenceAndFeatures</partinfo>
  

Revision as of 17:36, 11 October 2023


eGFP_simple premature stop codon

eGFP_premature stop codon is a protein coding sequence that contains the eGFP with a premature stop codon. This coding sequence is particularly useful for Traffic Light Reporter (TLR) assays that screen for DNA editing activity.

Usage and Biology

This sequence is used in a TLR assay for prime editing where pegRNAs and ngRNAs targeting the premature stop codon were designed to swap the premature stop codon into a functional amino acid, restoring the wild-type eGFP.

When cloned into a suitable plasmid and subsequently transfected into mammalian cells for in vitro DNA editing, successfully edited cells will fluorescence green which can then be analysed with flow cytometry.

Sequence and Features

The sequence contains the eGFP with a premature stop codon.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]