Difference between revisions of "Part:BBa K4759021:Design"

 
 
(One intermediate revision by one other user not shown)
Line 6: Line 6:
  
  
−
===Design Notes===
+
===Design===
 
Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria
 
Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria
  
Line 16: Line 16:
  
 
===References===
 
===References===
 +
[1] Sultana, N. Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids 2018, 136. DOI: 10.1016/j.steroids.2018.01.007.
 +
[2] Watchmaker, J.; Callaghan, D. J., III; Dover, J. S. Deoxycholic Acid in Aesthetic Medicine. Advances in Cosmetic Surgery 2020, 3 (1), 77-87. DOI: 10.1016/j.yacs.2020.01.009

Latest revision as of 17:14, 11 October 2023


hemB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 467
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 467
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 467
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 467
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria


Source

A gene coding for porphobilinogen synthase (PBGS),The enzyme catalyzes the asymmetric condensation and cyclization of two 5-aminolevulinate molecules

References

[1] Sultana, N. Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids 2018, 136. DOI: 10.1016/j.steroids.2018.01.007. [2] Watchmaker, J.; Callaghan, D. J., III; Dover, J. S. Deoxycholic Acid in Aesthetic Medicine. Advances in Cosmetic Surgery 2020, 3 (1), 77-87. DOI: 10.1016/j.yacs.2020.01.009