Difference between revisions of "Part:BBa K4759025:Design"

 
 
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===Design Notes===
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===Design===
 
Recombinant plasmid pCDFDuet-hemA-hemL is expressed in engineered bacteria
 
Recombinant plasmid pCDFDuet-hemA-hemL is expressed in engineered bacteria
  
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===References===
 
===References===
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[1]Sultana, N. Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids 2018, 136. DOI: 10.1016/j.steroids.2018.01.007.

Latest revision as of 17:07, 11 October 2023


hemL


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1028
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1028
    Illegal NheI site found at 738
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1028
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1028
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1028
    Illegal AgeI site found at 388
    Illegal AgeI site found at 721
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

Recombinant plasmid pCDFDuet-hemA-hemL is expressed in engineered bacteria


Source

A genecoding for glutamate-1-semialdehyde 2,1-aminomutase (GSAM).

References

[1]Sultana, N. Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids 2018, 136. DOI: 10.1016/j.steroids.2018.01.007.