Difference between revisions of "Part:BBa K4586028"
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This composite parts codes for lamp2b which is a transmembrane protein known as lysosome-associated membrane glycoprotein 2b, which is unique to the membrane of exosomes and its external domain conjugated to citrullinated vimentin to mediate its expression on the surface of the exosomes.  
 
This composite parts codes for lamp2b which is a transmembrane protein known as lysosome-associated membrane glycoprotein 2b, which is unique to the membrane of exosomes and its external domain conjugated to citrullinated vimentin to mediate its expression on the surface of the exosomes.  
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<html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              max-width:850px;
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width:100%;
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height:auto;
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position: relative;
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top: 50%;
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left: 50%;
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transform: translate( -50%);
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padding-bottom:25px;
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padding-top:25px;
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"src="https://static.igem.wiki/teams/4586/wiki/parts/c-v-part-zero.png
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">
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<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
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lang=EN style='font-size:11.0pt;line-height:115%'>Figure 1.  illustrates the structure of CCP1 within the extracellular domain of the Syn notch receptor expressed on the surface of the engineered MSC </span></p></div></html>
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<br><br>
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<html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              max-width:850px;
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width:100%;
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height:auto;
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position: relative;
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top: 50%;
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left: 50%;
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transform: translate( -50%);
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padding-bottom:25px;
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padding-top:25px;
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"src="https://static.igem.wiki/teams/4586/wiki/parts/part-0.png
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">
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<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
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lang=EN style='font-size:11.0pt;line-height:115%'>Figure 1: This figure illustrates the construction of our engineered exosomes that express citrullinated vimentin on their membranes conjugated to a transmembrane protein known as lysosome-associated membrane glycoprotein 2b (lamp2b), which is unique to the membrane of exosomes. </span></p></div></html>
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==literature characterization of ccp1==
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The study used gel electrophoresis to structurally characterize CCP1 compared to other parts. In Addition to studying its presence and association with neural tissues in different cross sections.
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<html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              max-width:850px;
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width:100%;
 +
height:auto;
 +
position: relative;
 +
top: 50%;
 +
left: 45%;
 +
transform: translate( -50%);
 +
padding-bottom:25px;
 +
padding-top:25px;
 +
"src="https://static.igem.wiki/teams/4586/wiki/math-mod/math-mod/embj-37-e100540-g005.jpg">
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<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
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lang=EN style='font-size:11.0pt;line-height:115%'>A)They observed ccp1 in proteins of brain, spinal cord and peripheral nerves.They confirmed that by detection of extra band in HEK293 transfected with ccp1-YFP fusion proteins and absence of signals in pcd mouse brain
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B)They found relation of ccp1 with myelinated axons(NF200) but not compact myelin (MBP)
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C)They found ccp1 in motor neurons( CHAT) in the ventral horn of spinal cord
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. </span></p></div></html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:46, 11 October 2023


CCP1 exosomes receptor

Usage and Description

This composite parts codes for lamp2b which is a transmembrane protein known as lysosome-associated membrane glycoprotein 2b, which is unique to the membrane of exosomes and its external domain conjugated to citrullinated vimentin to mediate its expression on the surface of the exosomes.

Figure 1. illustrates the structure of CCP1 within the extracellular domain of the Syn notch receptor expressed on the surface of the engineered MSC



Figure 1: This figure illustrates the construction of our engineered exosomes that express citrullinated vimentin on their membranes conjugated to a transmembrane protein known as lysosome-associated membrane glycoprotein 2b (lamp2b), which is unique to the membrane of exosomes.

literature characterization of ccp1

The study used gel electrophoresis to structurally characterize CCP1 compared to other parts. In Addition to studying its presence and association with neural tissues in different cross sections.

A)They observed ccp1 in proteins of brain, spinal cord and peripheral nerves.They confirmed that by detection of extra band in HEK293 transfected with ccp1-YFP fusion proteins and absence of signals in pcd mouse brain B)They found relation of ccp1 with myelinated axons(NF200) but not compact myelin (MBP) C)They found ccp1 in motor neurons( CHAT) in the ventral horn of spinal cord .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 670
    Illegal NotI site found at 679
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]