Difference between revisions of "Part:BBa K4645014"

 
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RelE is a toxin protein that inhibits translation. RNA thermosensors which could inhibit downstream gene expression when the temperature is 37 ℃ but not affect downstream gene expression significantly when the temperature is below 28 ℃.The combination of two components can regulate the expression of toxin proteins by changing the temperature, and then control the death of engineering bacteria.
 
RelE is a toxin protein that inhibits translation. RNA thermosensors which could inhibit downstream gene expression when the temperature is 37 ℃ but not affect downstream gene expression significantly when the temperature is below 28 ℃.The combination of two components can regulate the expression of toxin proteins by changing the temperature, and then control the death of engineering bacteria.
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<center><img src="https://static.igem.wiki/teams/4645/wiki/wet-lab/suicide/rnarele.png" style="width:50%; "></center>
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<center><b>Figure 1. Bacterial OD600 over time .</b> </center>
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Revision as of 15:31, 11 October 2023


Combination element of RNA thermosensors regulatory RelE toxin protein

RelE is a toxin protein that inhibits translation. RNA thermosensors which could inhibit downstream gene expression when the temperature is 37 ℃ but not affect downstream gene expression significantly when the temperature is below 28 ℃.The combination of two components can regulate the expression of toxin proteins by changing the temperature, and then control the death of engineering bacteria. 无标题文档


Figure 1. Bacterial OD600 over time .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 82