Difference between revisions of "Part:BBa K4645004"

Revision as of 15:27, 11 October 2023

VqmAphage

This part is meant to express vqmA gene which would translate into protein VqmA_Phage.
Vibrio cholerae uses a quorum-sensing (QS) system composed of the autoinducer 3,5-dimethylpyrazin-2-ol (DPO) and receptor VqmA, which together repress genes for virulence and biofilm formation. VqmA_Vc activates expression of the vqmR gene encoding the small regulatory RNA VqmR. Vibriophage VP882 encodes a VqmA homolog called VqmA_Phage that activates the qtip promoter (Pqtip). It’s worth noting that, VqmA_Phage can activate vqmR expression but VqmA_Vc cannot activate expression of qtip. Promoter selectivity is driven by each VqmA DNA-binding domain and key DNA sequences in the vqmR and qtip promoters are required to maintain specificity.[1]

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 535
1000
COMPATIBLE WITH RFC[1000]

Reference

[1] Silpe JE, Bassler BL. A Host-Produced Quorum-Sensing Autoinducer Controls a Phage Lysis-Lysogeny Decision. Cell. 2019 Jan 10;176(1-2):268-280.e13. [2] Duddy OP, Huang X, Silpe JE, Bassler BL. Mechanism underlying the DNA-binding preferences of the Vibrio cholerae and vibriophage VP882 VqmA quorum-sensing receptors. PLoS Genet. 2021 Jul 6;17(7):e1009550.

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 ===Reference===
+[1] Silpe JE, Bassler BL. A Host-Produced Quorum-Sensing Autoinducer Controls a Phage Lysis-Lysogeny Decision. Cell. 2019 Jan 10;176(1-2):268-280.e13.
+[2] Duddy OP, Huang X, Silpe JE, Bassler BL. Mechanism underlying the DNA-binding preferences of the Vibrio cholerae and vibriophage VP882 VqmA quorum-sensing receptors. PLoS Genet. 2021 Jul 6;17(7):e1009550.
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