Difference between revisions of "Part:BBa K4645004"

Revision as of 15:20, 11 October 2023

VqmAphage

This part is meant to express vqmA gene which would translate into protein VqmA_Phage.
Vibrio cholerae uses a quorum-sensing (QS) system composed of the autoinducer 3,5-dimethylpyrazin-2-ol (DPO) and receptor VqmA, which together repress genes for virulence and biofilm formation. VqmA_Vc activates expression of the vqmR gene encoding the small regulatory RNA VqmR. Vibriophage VP882 encodes a VqmA homolog called VqmA_Phage that activates the qtip promoter (Pqtip). It’s worth noting that, VqmA_Phage can activate vqmR expression but VqmA_Vc cannot activate expression of qtip. Promoter selectivity is driven by each VqmA DNA-binding domain and key DNA sequences in the vqmR and qtip promoters are required to maintain specificity. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 535
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4645004 short</partinfo>
-This part is meant to express vqmA gene which would translate into protein VqmA<sub>phage</sub>.<br>
+This part is meant to express vqmA gene which would translate into protein VqmA<sub>Phage</sub>.<br>
-Vibrio cholerae uses a quorum-sensing (QS) system composed of the autoinducer 3,5-dimethylpyrazin-2-ol (DPO) and receptor VqmA, which together repress genes for virulence and bioﬁlm formation. VqmAVc activates expression of the vqmR gene encoding the small regulatory RNA VqmR. Vibriophage VP882 encodes a VqmA homolog called VqmAPhage that activates the qtip promoter (Pqtip). It’s worth noting that, VqmAPhage can activate vqmR expression but VqmAVc cannot activate expression of qtip. Promoter selectivity is driven by each VqmA DNA-binding domain and key DNA sequences in the vqmR and qtip promoters are required to maintain specificity.
+Vibrio cholerae uses a quorum-sensing (QS) system composed of the autoinducer 3,5-dimethylpyrazin-2-ol (DPO) and receptor VqmA, which together repress genes for virulence and biofilm formation. VqmA<sub>Vc</sub> activates expression of the vqmR gene encoding the small regulatory RNA VqmR. Vibriophage VP882 encodes a VqmA homolog called VqmA<sub>Phage</sub> that activates the qtip promoter (Pqtip). It’s worth noting that, VqmA<sub>Phage</sub> can activate vqmR expression but VqmA<sub>Vc</sub> cannot activate expression of qtip. Promoter selectivity is driven by each VqmA DNA-binding domain and key DNA sequences in the vqmR and qtip promoters are required to maintain specificity.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===