Difference between revisions of "Part:BBa K4698007"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | While reviewing CRISPR-related materials, we discovered a particularly interesting evolutionary system- phage-assisted continuous evolution (PACE)[1,2], which can enable continuous directed evolution of gene-coding molecules that can be associated with protein production in <i>E. coli</i>. Then, we found a protein degradation tag-degron[3], that enables rapid hydrolysis of proteins with little functionality, which allows degron to be used in a number of protein co-expression screening systems. | + | <html> |
| + | <p>While reviewing CRISPR-related materials, we discovered a particularly interesting evolutionary system- phage-assisted continuous evolution (PACE)[1,2], which can enable continuous directed evolution of gene-coding molecules that can be associated with protein production in <i>E. coli</i>. Then, we found a protein degradation tag-degron[3], that enables rapid hydrolysis of proteins with little functionality, which allows degron to be used in a number of protein co-expression screening systems.</p> | ||
<img src="https://static.igem.wiki/teams/4698/wiki/improved-part-gfp.png" class= "center" style="width:500px"> | <img src="https://static.igem.wiki/teams/4698/wiki/improved-part-gfp.png" class= "center" style="width:500px"> | ||
| − | Figure 1, Testing the protein degradation efficiency of degron. blank, LB medium. NT, E.coli without GFP expression plasmid, n=3. | + | <p>Figure 1, Testing the protein degradation efficiency of degron. blank, LB medium. NT, E.coli without GFP expression plasmid, <i>n</i>=3.</p> |
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2. Miller, S. M., Wang, T. & Liu, D. R. Phage-assisted continuous and non-continuous evolution. <i>Nature Protocols</i> 15, 4101-4127 (2020). | 2. Miller, S. M., Wang, T. & Liu, D. R. Phage-assisted continuous and non-continuous evolution. <i>Nature Protocols</i> 15, 4101-4127 (2020). | ||
3. Karzai, A. W., Roche, E. D. & Sauer, R. T. The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue. <i>Nat Struct Biol</i> 7, 449-455 (2000). | 3. Karzai, A. W., Roche, E. D. & Sauer, R. T. The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue. <i>Nat Struct Biol</i> 7, 449-455 (2000). | ||
| − | + | </html> | |
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K4698007 parameters</partinfo> | <partinfo>BBa_K4698007 parameters</partinfo> | ||
Revision as of 15:20, 11 October 2023
GFP with a protein degradation tag attached
A protein degradation tag (degron) is attached to the C-terminus of GFP to promote protein degradation.
Usage and Biology
While reviewing CRISPR-related materials, we discovered a particularly interesting evolutionary system- phage-assisted continuous evolution (PACE)[1,2], which can enable continuous directed evolution of gene-coding molecules that can be associated with protein production in E. coli. Then, we found a protein degradation tag-degron[3], that enables rapid hydrolysis of proteins with little functionality, which allows degron to be used in a number of protein co-expression screening systems.
Figure 1, Testing the protein degradation efficiency of degron. blank, LB medium. NT, E.coli without GFP expression plasmid, n=3.