Difference between revisions of "Part:BBa K4880001:Design"

 
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===Design Notes===
 
===Design Notes===
We assembled it with Ptrc and Theophylline-dependent riboswitch.
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===Source===
 
===Source===
  
This sequence is derived from the Santalum album genome, and it is adapted to the cyanobacteria codon. We took advantage of the publication of Blanc-Garin et al. (2022).
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This sequence is derived from the Santalum album genome, and it is adapted to the cyanobacteria codon.
 
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===References===
 
===References===
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Blanc-Garin, V., Chenebault, C., Diaz-Santos, E., Vincent, M., Sassi, J.-F., Cassier-Chauvat, C., & Chauvat, F. (2022). Exploring the potential of the model cyanobacterium Synechocystis PCC 6803 for the photosynthetic production of various high-value terpenes. Biotechnology for Biofuels and Bioproducts, 15(1). https://doi.org/10.1186/s13068-022-02211-0

Latest revision as of 13:46, 11 October 2023


SaSS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 862
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 862
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 862
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 862
    Illegal AgeI site found at 823
    Illegal AgeI site found at 1462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1257


Design Notes

-


Source

This sequence is derived from the Santalum album genome, and it is adapted to the cyanobacteria codon.


References

Blanc-Garin, V., Chenebault, C., Diaz-Santos, E., Vincent, M., Sassi, J.-F., Cassier-Chauvat, C., & Chauvat, F. (2022). Exploring the potential of the model cyanobacterium Synechocystis PCC 6803 for the photosynthetic production of various high-value terpenes. Biotechnology for Biofuels and Bioproducts, 15(1). https://doi.org/10.1186/s13068-022-02211-0