Difference between revisions of "Part:BBa K4960015"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4960015 short</partinfo>
 
<partinfo>BBa_K4960015 short</partinfo>
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===Properties===
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===Profile===
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Name: pvc16<br>
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Base Pairs: 882bp<br>
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Origin: Photorhabdus<br>
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Properties: Pvc16 is a part of pvc sheath terminator.<br>
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<!-- Add more about the biology of this part here-->
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===Usage and Biology===
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)<br>
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<html>
  
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PVC16 is a part of PVC Sheath Terminator.
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<figure class="figure">
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded"  height="150px">
  
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<!-- Add more about the biology of this part here
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</figure>
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===Usage and Biology===
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Possessing a central core and six arms.
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</html>
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Six helices from the Pvc16 hexamer completely seal the central channel of the tube, leaving a narrow pore with a diameter of 7 A.
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Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
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Each Pvc16 monomer exhibits a dumbbell-like structure. The N-terminal domain (NTD) and C-terminal domain (CTD) is connected by a middle linker (MD).
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<br>
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The main function is to be as a tube terminator.
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Pvc16 is a tube terminator,which possesses a central core and six arms.Six helices from the Pvc16 hexamer completely seal the central channel of the tube, leaving a narrow pore with a diameter of 7 A.Each Pvc16 monomer exhibits a dumbbell-like structure. Each Pvc16-NTD interacts with three copies of Pvc1.Pvc16-CTD stabilizes the sheath-tube complex at the capping end.The N-terminal domain (NTD) and C-terminal domain (CTD) is connected by a middle linker (MD).<br>
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Each Pvc16-NTD interacts with three copies of Pvc1.
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Significantly,the binding of Pvc16 onto the PVC complex might not require the help of outer sheath and the capping of Pvc16 on the distal Pvc1 ring might set the signal to initiate the sheath loading.<br>
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In the absence of Pvc16 (DPvc16), the baseplate and tube could still form, but not the sheath, and the length of the tube appears to be much larger.
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After the tube reaches optimal length (likely controlled by the tape measure protein Pvc14), Pvc16 interacts with the topmost inner tube layer to terminate the tube elongation.<br>
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A minor role of Pvc16-CTD in stabilizing the sheath-tube complex at the capping end.
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The binding of Pvc16 onto the PVC complex might not require the help of outer sheath and the capping of Pvc16 on the distal Pvc1 ring might set the signal to initiate the sheath loading.
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After the tube reaches optimal length (likely controlled by the tape measure protein Pvc14), Pvc16 interacts with the topmost inner tube layer to terminate the tube elongation.
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===Sequence and Features===
 
===Sequence and Features===
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<!-- Uncomment this to enable Functional Parameter display -->
 
<!-- Uncomment this to enable Functional Parameter display -->
 
===References===
 
===References===
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[1] J. Kreitz et al., “Programmable protein delivery with a bacterial contractile injection system,” Nature, vol. 616, no. 7956, pp. 357–364, Apr. 2023, doi: 10.1038/s41586-023-05870-7.<br>
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[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br>
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[2] F. Jiang et al., “Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System,” Cell, vol. 177, no. 2, pp. 370-383.e15, Apr. 2019, doi: 10.1016/j.cell.2019.02.020.
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[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br>

Revision as of 13:12, 11 October 2023

pvc16

Profile

Name: pvc16
Base Pairs: 882bp
Origin: Photorhabdus
Properties: Pvc16 is a part of pvc sheath terminator.

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

Pvc16 is a tube terminator,which possesses a central core and six arms.Six helices from the Pvc16 hexamer completely seal the central channel of the tube, leaving a narrow pore with a diameter of 7 A.Each Pvc16 monomer exhibits a dumbbell-like structure. Each Pvc16-NTD interacts with three copies of Pvc1.Pvc16-CTD stabilizes the sheath-tube complex at the capping end.The N-terminal domain (NTD) and C-terminal domain (CTD) is connected by a middle linker (MD).
Significantly,the binding of Pvc16 onto the PVC complex might not require the help of outer sheath and the capping of Pvc16 on the distal Pvc1 ring might set the signal to initiate the sheath loading.
After the tube reaches optimal length (likely controlled by the tape measure protein Pvc14), Pvc16 interacts with the topmost inner tube layer to terminate the tube elongation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 647
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.