Difference between revisions of "Part:BBa K4583053"

 
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PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz
 
PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz
−
 
+
==Usage and Biology==
 +
===1. Three-layer Dynamic Regulation Model===
 +
===2. The first layer--Growth control===
 +
===3. The second layer--Production control===
 +
===4. The Third layer--Product-release control===
 +
==Characterization==
 +
===1. Protocols===
 +
===2. The Characterization of PesaS and PesaRwt/PesaRc/PesaRp===
 +
===3. The Characterization of PesaRwt/PesaRc/PesaRp and PYU3/PYU7/PYU16/PYU92===
 +
==Summary of Results==
 +
==Our part provides an example for future iGEM team==
 +
==Limitations==
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:52, 11 October 2023


PHB production Regulation: PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz

PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz

Usage and Biology

1. Three-layer Dynamic Regulation Model

2. The first layer--Growth control

3. The second layer--Production control

4. The Third layer--Product-release control

Characterization

1. Protocols

2. The Characterization of PesaS and PesaRwt/PesaRc/PesaRp

3. The Characterization of PesaRwt/PesaRc/PesaRp and PYU3/PYU7/PYU16/PYU92

Summary of Results

Our part provides an example for future iGEM team

Limitations

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2740
    Illegal BglII site found at 3565
    Illegal BamHI site found at 1791
    Illegal XhoI site found at 1511
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2046
    Illegal NgoMIV site found at 2117
    Illegal NgoMIV site found at 2717
    Illegal NgoMIV site found at 3029
    Illegal NgoMIV site found at 3308
    Illegal NgoMIV site found at 3960
    Illegal NgoMIV site found at 3982
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5826
    Illegal SapI.rc site found at 769