Difference between revisions of "Part:BBa K4960011"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4960011 short</partinfo>
 
<partinfo>BBa_K4960011 short</partinfo>
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===Properties===
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===Profile===
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Name: pvc11<br>
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Base Pairs: 2187bp<br>
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Origin: Photorhabdus<br>
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Properties: Pvc11 is a part of pvc baseplate.<br>
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<!-- Add more about the biology of this part here-->
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===Usage and Biology===
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)<br>
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<html>
  
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PVC11 is a part of PVC Baseplate.
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<figure class="figure">
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded"  height="150px">
  
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<!-- Add more about the biology of this part here
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</figure>
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===Usage and Biology===
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In the assembled wedges, six copies of Pvc11-Pvc12 heterodimers form a hexagonal ring through the dimerization between the domain V of Pvc11 and the domain III of Pvc12 from a neighboring heterodimer.
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</html>
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Using an extrusion for the interactions with adjacent domains with PVC11.
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Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
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One part involved in the association between the wedges and the central hub.
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<br>
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In the assembled wedges, six copies of Pvc11-Pvc12 heterodimers form a hexagonal ring through the dimerization between the domain V of pvc11 and the domain III of pvc12 from a neighboring heterodimer.<br>
 +
Using an extrusion for the interactions with adjacent domains with pvc11.<br>
 +
One part involved in the association between the wedges and the central hub.<br>
 
<!-- -->
 
<!-- -->
 
===Sequence and Features===
 
===Sequence and Features===
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<!-- Uncomment this to enable Functional Parameter display -->
 
<!-- Uncomment this to enable Functional Parameter display -->
 
===References===
 
===References===
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[1] J. Kreitz et al., “Programmable protein delivery with a bacterial contractile injection system,” Nature, vol. 616, no. 7956, pp. 357–364, Apr. 2023, doi: 10.1038/s41586-023-05870-7.<br>
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[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br>
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[2] F. Jiang et al., “Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System,” Cell, vol. 177, no. 2, pp. 370-383.e15, Apr. 2019, doi: 10.1016/j.cell.2019.02.020.
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[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br>

Revision as of 12:44, 11 October 2023


pvc11

Profile

Name: pvc11
Base Pairs: 2187bp
Origin: Photorhabdus
Properties: Pvc11 is a part of pvc baseplate.

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

In the assembled wedges, six copies of Pvc11-Pvc12 heterodimers form a hexagonal ring through the dimerization between the domain V of pvc11 and the domain III of pvc12 from a neighboring heterodimer.
Using an extrusion for the interactions with adjacent domains with pvc11.
One part involved in the association between the wedges and the central hub.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 891
    Illegal AgeI site found at 1577
    Illegal AgeI site found at 1718
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.