Difference between revisions of "Part:BBa K4960008"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4960008 short</partinfo>
 
<partinfo>BBa_K4960008 short</partinfo>
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===Properties===
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===Profile===
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Name: PVC8<br>
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PVC8 is a part of PVC Spike.
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Base Pairs: 1617bp<br>
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Origin: Photorhabdus<br>
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Properties: PVC8 is a part of PVC Spike.<br>
 
<!-- Add more about the biology of this part here-->
 
<!-- Add more about the biology of this part here-->
 
===Usage and Biology===
 
===Usage and Biology===
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)<br>
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<figure class="figure">
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded"  height="150px">
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</figure>
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</html>
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Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
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<br>
 
A part of the simplified architecture of T4 phage baseplate lying in the PVC syringe with PVC5, PVC7 and PVC10.  
 
A part of the simplified architecture of T4 phage baseplate lying in the PVC syringe with PVC5, PVC7 and PVC10.  
 
One part involved in the association between the wedges and the central hub.  
 
One part involved in the association between the wedges and the central hub.  
 
A fusion protein of T4 gp5 and gp27 homologs.
 
A fusion protein of T4 gp5 and gp27 homologs.
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Two sides of hexameric rings are found opposite surface charges, PVC8 included
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Two sides of hexameric rings are found opposite surface charges, PVC8 included.
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4960008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4960008 SequenceAndFeatures</partinfo>
  
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===References===
 
===References===
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[1]J. Kreitz et al., “Programmable protein delivery with a bacterial contractile injection system,” Nature, vol. 616, no. 7956, pp. 357–364, Apr. 2023.<br>
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[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br>
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[2] F. Jiang et al., “Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System,” Cell, vol. 177, no. 2, pp. 370-383.e15, Apr. 2019;
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[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br>

Revision as of 12:26, 11 October 2023


pvc8

Profile

Name: PVC8
Base Pairs: 1617bp
Origin: Photorhabdus
Properties: PVC8 is a part of PVC Spike.

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

A part of the simplified architecture of T4 phage baseplate lying in the PVC syringe with PVC5, PVC7 and PVC10. One part involved in the association between the wedges and the central hub. A fusion protein of T4 gp5 and gp27 homologs. Two sides of hexameric rings are found opposite surface charges, PVC8 included.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1213
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 511
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.