Difference between revisions of "Part:BBa K4800047"
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− | + | We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene GabT to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-GabT. | |
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Latest revision as of 12:02, 11 October 2023
pTrc99a-YahK-GabT
We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene GabT to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-GabT.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3624
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4039
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5172
Illegal BsaI site found at 6240
Illegal SapI.rc site found at 767