Difference between revisions of "Part:BBa K4800051"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4800051 short</partinfo>
 
<partinfo>BBa_K4800051 short</partinfo>
  
−
To compare the activity of different transaminases, the DNA fragments of Ptrc-RBS-YahK were amplified and ligated into the plasmids of pTrc99a-SpuC.
+
We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene SpuC to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-SpuC.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 11:33, 11 October 2023


pTrc99a-YahK-SpuC

We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene SpuC to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-SpuC.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4846
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4846
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3624
    Illegal XhoI site found at 5140
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4846
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4846
    Illegal AgeI site found at 4129
    Illegal AgeI site found at 4582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5346
    Illegal BsaI site found at 6414
    Illegal SapI.rc site found at 767