Difference between revisions of "Part:BBa K4613303"
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<partinfo>BBa_K4613303 short</partinfo> | <partinfo>BBa_K4613303 short</partinfo> | ||
− | In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried the T7 <em>lac</em> promoter from pET-29a (+). | + | In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried the T7 <em>lac</em> promoter from pET-29a(+). |
The composite part can be directly imported into plasmid and express T3-ADH3 induced with IPTG. | The composite part can be directly imported into plasmid and express T3-ADH3 induced with IPTG. | ||
Revision as of 11:28, 11 October 2023
pET-29a(+)-T3-ADH3
In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried the T7 lac promoter from pET-29a(+). The composite part can be directly imported into plasmid and express T3-ADH3 induced with IPTG.
Reference
- Dai Z, Yang X, Wu F, et al.Living fabrication of functional semi-interpenetrating polymeric materials[J].Nat Commun,2021, 12 (1): 3422.
- Zakeri B, Fierer J O, Celik E, et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proc Natl Acad Sci U S A,2012, 109 (12): E690-7.
- Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.
- Dai L, Niu D, Huang J W, et al.Cryo-EM structure and rational engineering of a superefficient ochratoxin A-detoxifying amidohydrolase[J].J Hazard Mater,2023, 458: 131836.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1007
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1742
Illegal AgeI site found at 1430
Illegal AgeI site found at 1592 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 75