Difference between revisions of "Part:BBa K4613303"

(Reference)
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<partinfo>BBa_K4613303 short</partinfo>
 
<partinfo>BBa_K4613303 short</partinfo>
  
In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried the T7 <em>lac</em> promoter from pET-29a (+).
+
In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried the T7 <em>lac</em> promoter from pET-29a(+).
 
The composite part can be directly imported into plasmid and express T3-ADH3 induced with IPTG.
 
The composite part can be directly imported into plasmid and express T3-ADH3 induced with IPTG.
  

Revision as of 11:28, 11 October 2023


pET-29a(+)-T3-ADH3

In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried the T7 lac promoter from pET-29a(+). The composite part can be directly imported into plasmid and express T3-ADH3 induced with IPTG.

Reference

  1. Dai Z, Yang X, Wu F, et al.Living fabrication of functional semi-interpenetrating polymeric materials[J].Nat Commun,2021, 12 (1): 3422.
  2. Zakeri B, Fierer J O, Celik E, et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proc Natl Acad Sci U S A,2012, 109 (12): E690-7.
  3. Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.
  4. Dai L, Niu D, Huang J W, et al.Cryo-EM structure and rational engineering of a superefficient ochratoxin A-detoxifying amidohydrolase[J].J Hazard Mater,2023, 458: 131836.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1007
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1742
    Illegal AgeI site found at 1430
    Illegal AgeI site found at 1592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 75