Difference between revisions of "Part:BBa K4800048"

 
 
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<partinfo>BBa_K4800048 short</partinfo>
 
<partinfo>BBa_K4800048 short</partinfo>
  
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To compare the activity of different transaminases, the DNA fragments of Ptrc-RBS-YahK were amplified and ligated into the plasmids of pTrc99a-PatA.
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We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene PatA to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-PatA.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 10:41, 11 October 2023


pTrc99a-YahK-PatA

We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene PatA to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-PatA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3624
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3946
    Illegal AgeI site found at 4453
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5331
    Illegal BsaI site found at 6399
    Illegal SapI.rc site found at 767