Difference between revisions of "Part:BBa K4907020"
Line 8: | Line 8: | ||
===Usage and design=== | ===Usage and design=== | ||
− | We used This part to combine hrpR(<partinfo>BBa_K4907021</partinfo>) and hrpS(<partinfo>BBa_K4907022</partinfo>) to construct the <i>hrp</i> system and obtained the composite part <partinfo>BBa_K4907126</partinfo>, which are assembled on the expression vector pSB1C3(Fig. 1). | + | We used This part to combine <i>hrpR</i>(<partinfo>BBa_K4907021</partinfo>) and <i>hrpS</i>(<partinfo>BBa_K4907022</partinfo>) to construct the <i>hrp</i> system and obtained the composite part <partinfo>BBa_K4907126</partinfo>, which are assembled on the expression vector pSB1C3(Fig. 1). |
<center><html><img src="https://static.igem.wiki/teams/4907/wiki/parts/haoxihuan/i0500-b0034-hrpr-b0015-arabad-promoter-b0034-hrps-b0015.png" width="700px"></html></center> | <center><html><img src="https://static.igem.wiki/teams/4907/wiki/parts/haoxihuan/i0500-b0034-hrpr-b0015-arabad-promoter-b0034-hrps-b0015.png" width="700px"></html></center> | ||
<center><b>Fig. 1 Gene circuits of the composite part BBa_K4907126</b></center> | <center><b>Fig. 1 Gene circuits of the composite part BBa_K4907126</b></center> |
Latest revision as of 10:38, 11 October 2023
araBAD promoter
Biology
The araBAD promoter is an E. coli promoter that is tightly controlled by inducer: L-arabinose.
Usage and design
We used This part to combine hrpR(BBa_K4907021) and hrpS(BBa_K4907022) to construct the hrp system and obtained the composite part BBa_K4907126, which are assembled on the expression vector pSB1C3(Fig. 1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 300
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 239
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 74
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 56