Difference between revisions of "Part:BBa K4613024"
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<partinfo>BBa_K4613024 short</partinfo> | <partinfo>BBa_K4613024 short</partinfo> | ||
| − | The composite part was constructed to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency. Moreover, we hope Mature Carboxypeptidase A (M-CPA), which can be found under https://parts.igem.org/Part:BBa_K4613013, | + | The composite part was constructed to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency. Moreover, we hope Mature Carboxypeptidase A (M-CPA), which can be found under https://parts.igem.org/Part:BBa_K4613013, can be expressed successfully in <em>E. coli</em> Nissle 1917 (EcN). We tried T5 <em>lac</em> promoter from (pQE-80L). |
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Revision as of 10:14, 11 October 2023
pQE-80L-T3-MCPA
The composite part was constructed to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency. Moreover, we hope Mature Carboxypeptidase A (M-CPA), which can be found under https://parts.igem.org/Part:BBa_K4613013, can be expressed successfully in E. coli Nissle 1917 (EcN). We tried T5 lac promoter from (pQE-80L).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1617
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]