Difference between revisions of "Part:BBa K4613303"

 
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<partinfo>BBa_K4613303 short</partinfo>
 
<partinfo>BBa_K4613303 short</partinfo>
  
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In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried the T7lac promoter from pET-29a+.
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In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried the T7 <em>lac</em> promoter from pET-29a+.
 
The composite part can be directly imported into plasmid and express T3-ADH3 induced with IPTG.
 
The composite part can be directly imported into plasmid and express T3-ADH3 induced with IPTG.
  

Revision as of 09:27, 11 October 2023


pET-29a(+)-T3-ADH3

In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried the T7 lac promoter from pET-29a+. The composite part can be directly imported into plasmid and express T3-ADH3 induced with IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1007
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1742
    Illegal AgeI site found at 1430
    Illegal AgeI site found at 1592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 75