Difference between revisions of "Part:BBa K4604006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Change of start codon to adjust to E. coli. Additional site directed mutagenesis to result in a stop codon. No | + | Change of start codon to adjust to <i>E. coli</i>. Additional site directed mutagenesis to result in a stop codon. No Methyonine triplet close to the inserted stop codon, translation should certainly end abruptly. |
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===Source=== | ===Source=== | ||
| − | Taken from Mycobacterium Smegmatis, synthesized by IDT. | + | Taken from <i>Mycobacterium Smegmatis</i>, sequence was taken from the National Library of Medicine (gene ID:61603312), synthesized by IDT. |
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Latest revision as of 09:09, 11 October 2023
bluB mutated
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Change of start codon to adjust to E. coli. Additional site directed mutagenesis to result in a stop codon. No Methyonine triplet close to the inserted stop codon, translation should certainly end abruptly.
Source
Taken from Mycobacterium Smegmatis, sequence was taken from the National Library of Medicine (gene ID:61603312), synthesized by IDT.