Difference between revisions of "Part:BBa K4800042"

Latest revision as of 08:47, 11 October 2023

pTrc99a-Spo3471

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene Spo3471 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-Spo3471.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 4051
Illegal PstI site found at 2666
Illegal PstI site found at 4063
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 2666
Illegal PstI site found at 4063
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4045
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 4051
Illegal PstI site found at 2666
Illegal PstI site found at 4063
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 4051
Illegal PstI site found at 2666
Illegal PstI site found at 4063
Illegal NgoMIV site found at 3326
Illegal AgeI site found at 3313
Illegal AgeI site found at 3437
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 4247
Illegal BsaI site found at 5315
Illegal SapI.rc site found at 767

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4800042 short</partinfo>
-Aspartate transaminase from <i>Ruegeria pomeroyi</i>. A pyridoxal-phosphate protein. Also acts on L-tyrosine, L-phenylalanine and L-tryptophan. Aspartate transaminase activity can be formed from the aromatic-amino-acid transaminase (EC 2.6.1.57) of Escherichia coli by controlled proteolysis , some EC 2.6.1.57 activity can be found in this enzyme from other sources ; indeed the enzymes are identical in Trichomonas vaginalis .
+We linearized the pTrc99a vector using restriction endonuclease and connected the target gene Spo3471 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-Spo3471.
 <!-- Add more about the biology of this part here