Difference between revisions of "Part:BBa K4800040"

 
 
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<partinfo>BBa_K4800040 short</partinfo>
 
<partinfo>BBa_K4800040 short</partinfo>
  
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NADPH-aldehyde reductase,from <i>Escherichia coli</i>.In addition,yahK is involved in biofilm formation by a reduced-genome E. coli strain
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We linearized the pTrc99a vector using restriction endonuclease and connected the target gene YahK to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK.
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 08:35, 11 October 2023


pTrc99a-YahK

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene YahK to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3627
    Illegal PstI site found at 3639
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3639
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3621
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3627
    Illegal PstI site found at 3639
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3627
    Illegal PstI site found at 3639
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3823
    Illegal BsaI site found at 4891
    Illegal SapI.rc site found at 767