Difference between revisions of "Part:BBa K4800039"

 
 
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<partinfo>BBa_K4800039 short</partinfo>
 
<partinfo>BBa_K4800039 short</partinfo>
  
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Alcohol dehydrogenase from <i>Escherichia coli</i>.A zinc protein. Acts on primary or secondary alcohols or hemi-acetals with very broad specificity.The  enzyme acts also on cyclic secondary alcohols.
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We linearized the pTrc99a vector using restriction endonuclease and connected the target gene Adhp to it through homologous recombination to obtain the recombinant plasmid pTrc99a-Adhp.
  
  

Latest revision as of 08:31, 11 October 2023


pTrc99a-Adhp

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene Adhp to it through homologous recombination to obtain the recombinant plasmid pTrc99a-Adhp.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3602
    Illegal PstI site found at 2582
    Illegal PstI site found at 3614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2582
    Illegal PstI site found at 3614
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3596
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3602
    Illegal PstI site found at 2582
    Illegal PstI site found at 3614
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3602
    Illegal PstI site found at 2582
    Illegal PstI site found at 3614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3798
    Illegal BsaI site found at 4866
    Illegal SapI.rc site found at 767