Difference between revisions of "Part:BBa K4613888"

 
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<partinfo>BBa_K4613888 short</partinfo>
 
<partinfo>BBa_K4613888 short</partinfo>
  
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This composite part confers the ability for engineered bacteria to undergo self-destruction when perceiving the high cell density. When we add IPTG,traI will be expressed and take charge of the formation of ligand, then traR, will bind to the ligand and become active and combine to <em>Ptra</em>, increasing the expression of downstream <em>lysis E</em>.
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This composite part confers the ability for engineered bacteria to undergo self-destruction when perceiving the high cell density. When we add IPTG,traI protein will be expressed and take charge of the formation of ligand, then traR, will bind to the ligand and become active and combine to <em>Ptra</em>, increasing the expression of downstream <em>lysis E</em>.
  
  

Revision as of 07:53, 11 October 2023


pSB1C3-tra-lysis

This composite part confers the ability for engineered bacteria to undergo self-destruction when perceiving the high cell density. When we add IPTG,traI protein will be expressed and take charge of the formation of ligand, then traR, will bind to the ligand and become active and combine to Ptra, increasing the expression of downstream lysis E.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 429
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 429
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 429
    Illegal BglII site found at 14
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 429
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 429
    Illegal NgoMIV site found at 1592
  • 1000
    COMPATIBLE WITH RFC[1000]