Difference between revisions of "Part:BBa K4743002"

Latest revision as of 07:39, 11 October 2023

NadA+ADH1 terminator with adapter

NadA gene codes for quinolinate synthase, which turns iminoaspartate into quinolate. The whole pathway is shown below:

NAD biosynthesis, iminoaspartate => quinolinate => NAD

Length checking of nadA

We use electrophoresis to check the length of the fragment

Figure 1. The length of NadA.

Overall metabolic pathway of NMN

NadA is involved in the pathway of Astaxanthin production in PTSH-Taiwan's project.

Figure 2. The Matabolic pathway of NMN production.

References

1.Fagnocchi, L., Biolchi, A., Ferlicca, F., Boccadifuoco, G., Brunelli, B., Brier, S., Norais, N., Chiarot, E., Bensi, G., Kroll, J. S., Pizza, M., Donnelly, J., Giuliani, M. M., & Delany, I. (2013). Transcriptional regulation of the nadA gene in Neisseria meningitidis impacts the prediction of coverage of a multicomponent meningococcal serogroup B vaccine. Infection and immunity, 81(2), 560–569. https://doi.org/10.1128/IAI.01085-12

2.Michalowski, C. B., Flachmann, R., Löffelhardt, W., & Bohnert, H. J. (1991). Gene nadA, Encoding Quinolinate Synthetase, Is Located on the Cyanelle DNA from Cyanophora paradoxa. Plant physiology, 95(1), 329–330. https://doi.org/10.1104/pp.95.1.329

3.Christine B. Michalowski, Ralf Flachmann, Wolfgang Löffelhardt, Hans J. Bohnert, Gene nadA, Encoding Quinolinate Synthetase, Is Located on the Cyanelle DNA from Cyanophora paradoxa , Plant Physiology, Volume 95, Issue 1, January 1991, Pages 329–330, https://doi.org/10.1104/pp.95.1.329

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1284
Illegal SpeI site found at 1089
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1284
Illegal SpeI site found at 1089
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1284
Illegal BamHI site found at 16
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1284
Illegal SpeI site found at 1089
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1284
Illegal SpeI site found at 1089
Illegal AgeI site found at 1297
1000
COMPATIBLE WITH RFC[1000]

@@ Line 12: / Line 12: @@
 We use electrophoresis to check the length of the fragment
-<html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/nada-nadb-length.png" </html>
+<html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/nada-nadb-nade-length.png" </html>
 <center>Figure 1. The length of NadA.</center>
@@ Line 23: / Line 23: @@
 <center>Figure 2. The Matabolic pathway of NMN production.</center>
+===References===
+.Fagnocchi, L., Biolchi, A., Ferlicca, F., Boccadifuoco, G., Brunelli, B., Brier, S., Norais, N., Chiarot, E., Bensi, G., Kroll, J. S., Pizza, M., Donnelly, J., Giuliani, M. M., & Delany, I. (2013). Transcriptional regulation of the nadA gene in Neisseria meningitidis impacts the prediction of coverage of a multicomponent meningococcal serogroup B vaccine. Infection and immunity, 81(2), 560–569. https://doi.org/10.1128/IAI.01085-12
+.Michalowski, C. B., Flachmann, R., Löffelhardt, W., & Bohnert, H. J. (1991). Gene nadA, Encoding Quinolinate Synthetase, Is Located on the Cyanelle DNA from Cyanophora paradoxa. Plant physiology, 95(1), 329–330. https://doi.org/10.1104/pp.95.1.329
+.Christine B. Michalowski, Ralf Flachmann, Wolfgang Löffelhardt, Hans J. Bohnert, Gene nadA, Encoding Quinolinate Synthetase, Is Located on the Cyanelle DNA from Cyanophora paradoxa  , Plant Physiology, Volume 95, Issue 1, January 1991, Pages 329–330, https://doi.org/10.1104/pp.95.1.329
 <!-- Add more about the biology of this part here
 ===Usage and Biology===