Difference between revisions of "Part:BBa K4613097"
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<partinfo>BBa_K4613097 short</partinfo> | <partinfo>BBa_K4613097 short</partinfo> | ||
| − | IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. | + | IPTG (Isopropylthiogalactoside) binds to <em>LacI</em> and inhibits its operation, therefore promoting transcription. |
This part additionally includes the <em>lac</em> operator(symmetric), which was optimized for tight binding of <em>lac</em> repressor to avoid the leakage of the downstream gene. | This part additionally includes the <em>lac</em> operator(symmetric), which was optimized for tight binding of <em>lac</em> repressor to avoid the leakage of the downstream gene. | ||
Latest revision as of 07:26, 11 October 2023
Plac
IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. This part additionally includes the lac operator(symmetric), which was optimized for tight binding of lac repressor to avoid the leakage of the downstream gene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 60
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 60
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 60
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 60
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 60
- 1000COMPATIBLE WITH RFC[1000]