Difference between revisions of "Part:BBa K4613216"

Revision as of 07:23, 11 October 2023

traI

The tra system in Agrobacterium tumefaciens is a LuxR/LuxI-type quorum sensing system. TraI protein facilitates the formation of ligand 3-oxo-C8-HSL(3OC8), and TraR, when bound to 3OC8, becomes active and combines to Ptra, increasing its transcription efficiency.

Reference

Fuqua C, Winans S C.Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes[J].J Bacteriol,1996, 178 (2): 435-40.
White C E, Winans S C.The quorum-sensing transcription factor TraR decodes its DNA binding site by direct contacts with DNA bases and by detection of DNA flexibility[J].Mol Microbiol,2007, 64 (1): 245-56.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 401
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4613216 short</partinfo>
-The tra system in Agrobacterium tumefaciens is a <em>LuxR/LuxI</em>-type quorum sensing system.
+The tra system in <em>Agrobacterium tumefaciens</em> is a <em>LuxR/LuxI</em>-type quorum sensing system.
 TraI protein facilitates the formation of ligand 3-oxo-C8-HSL(3OC8), and TraR, when bound to 3OC8, becomes active and combines to <em>Ptra</em>, increasing its transcription efficiency.
 ==== Reference ====
-#Fuqua WC, Winans SC. A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite. J Bacteriol. 1994 May;176(10):2796-806. doi: 10.1128/jb.176.10.2796-2806.1994. PMID: 8188582; PMCID: PMC205432.
+#Fuqua C, Winans S C.Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes[J].J Bacteriol,1996, 178 (2): 435-40.
-#White CE, Winans SC. The quorum-sensing transcription factor TraR decodes its DNA binding site by direct contacts with DNA bases and by detection of DNA flexibility. Mol Microbiol. 2007 Apr;64(1):245-56. doi: 10.1111/j.1365-2958.2007.05647.x. PMID: 17376086.
+#White C E, Winans S C.The quorum-sensing transcription factor TraR decodes its DNA binding site by direct contacts with DNA bases and by detection of DNA flexibility[J].Mol Microbiol,2007, 64 (1): 245-56.
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