Difference between revisions of "Part:BBa K4800019"
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We constructed EutM-SpyCatcher building blocks and attached itself covalently via isopeptide bond formation by the SpyTag-SpyCatcher system to the formed scaffolds,joining with enzyme elements to form a complex and improving the stability of the enzyme element. | We constructed EutM-SpyCatcher building blocks and attached itself covalently via isopeptide bond formation by the SpyTag-SpyCatcher system to the formed scaffolds,joining with enzyme elements to form a complex and improving the stability of the enzyme element. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 06:10, 11 October 2023
EutM-SpyCatcher
We constructed EutM-SpyCatcher building blocks and attached itself covalently via isopeptide bond formation by the SpyTag-SpyCatcher system to the formed scaffolds,joining with enzyme elements to form a complex and improving the stability of the enzyme element.
Build:
We amplified EutM-SpyCatcher-containing fragments from lab-conserved strains.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 42
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 42
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 42
Illegal BamHI site found at 36 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 42
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 42
- 1000COMPATIBLE WITH RFC[1000]