Difference between revisions of "Part:BBa K4800013"
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− | SgRNA, | + | SgRNA, which is small guide RNA, is an important component of the CRISPR knockdown system. The N20 sequence of the YcjQ SgRNA is derived from the YcjQ gene and can be localised to the acka-pta gene in the <i>Escherichia coli</i> NT1003 genome to assist the Cas9 protein in silencing the gene. |
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<strong>Fig.1 YcjQ sgRNA</strong> | <strong>Fig.1 YcjQ sgRNA</strong> | ||
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− | <p> | + | <p>We designed the N20 sequence of YcjQ by using the CHOPCHOP website (http://chopchop.cbu.uib.no), synthesized them on primers and ligated the N20 into the pTarget-sgRNA plasmid by means of PCR. |
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Revision as of 05:55, 11 October 2023
YcjQ sgRNA
SgRNA, which is small guide RNA, is an important component of the CRISPR knockdown system. The N20 sequence of the YcjQ SgRNA is derived from the YcjQ gene and can be localised to the acka-pta gene in the Escherichia coli NT1003 genome to assist the Cas9 protein in silencing the gene.
Build:
Fig.1 YcjQ sgRNA
We designed the N20 sequence of YcjQ by using the CHOPCHOP website (http://chopchop.cbu.uib.no), synthesized them on primers and ligated the N20 into the pTarget-sgRNA plasmid by means of PCR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]