Difference between revisions of "Part:BBa K4765014"
(→Usage and Biology) |
Siliang Zhan (Talk | contribs) (→Introduction) |
||
| Line 10: | Line 10: | ||
| <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/t-fudan-maa-pathway-wyj.png" alt="contributed by Fudan iGEM 2023"></html> | | <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/t-fudan-maa-pathway-wyj.png" alt="contributed by Fudan iGEM 2023"></html> | ||
|- | |- | ||
| − | | ''' | + | | '''Figure 1. The biosynthetic pathway of shinorine, porphyra-334, palythine-Ser, and palythine-Thr. 1''' |
|} | |} | ||
Revision as of 05:50, 11 October 2023
MysH codon optimized
Introduction
Nonheme iron-(II)- and 2oxoglutarate-dependent (Fe/2OG) oxygenase gene MysH completes the production of palythines from disubstituted MAAs[1].
|
| Figure 1. The biosynthetic pathway of shinorine, porphyra-334, palythine-Ser, and palythine-Thr. 1 |
Usage and Biology
We performed codon optimization on BBa_K4273014(NlmysH) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysH catalyzes the final reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 23
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.