Difference between revisions of "Part:BBa K4800012"
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     <p style="font-size: 150%; font-weight: bold;">Build:
 
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     <p><img src="https://static.igem.wiki/teams/4800/wiki/parts/acka-pta-sgrna.png" width="80%" height="80%"></p>
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     <p><img src="https://static.igem.wiki/teams/4800/wiki/parts/acka-pta-sgrna.png" width="50%" height="50%"></p>
 
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         <strong>Fig1. ackA-pta sgRNA</strong>
 
         <strong>Fig1. ackA-pta sgRNA</strong>

Revision as of 05:49, 11 October 2023


ackA-pta sgRNA

SgRNA, which is small guide RNA, is an important component of the CRISPR knockdown system. The N20 sequence of the ackA-pta SgRNA was derived from the ackA and pta genes and could be localised to the acka-pta gene in the Escherichia coli NT1003 genome to assist the Cas9 protein in silencing the gene.


Document

Build:

Fig1. ackA-pta sgRNA

We designed the N20 sequence of ackA-pta by using the CHOPCHOP website (http://chopchop.cbu.uib.no), synthesized them on primers and ligated the N20 into the pTarget-sgRNA plasmid by means of PCR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]