Difference between revisions of "Part:BBa K4800010"
 
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<partinfo>BBa_K4800010 short</partinfo>
 
<partinfo>BBa_K4800010 short</partinfo>
  
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ackA-pta Donor is exogenous DNA used by cells as a template for homologous recombination repair (HDR) after SgRNA and Cas9 complexes have caused DNA double-strand breaks at the target site.
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AckA-pta Donor is exogenous DNA used by cells as a template for homologous recombination repair (HDR) after SgRNA and Cas9 complexes have caused DNA double-strand breaks at the target site.
  
 
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     <p style="font-size: 180%; font-weight: bold;">Build:
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     <p style="font-size: 150%; font-weight: bold;">Build:
 
      
 
      
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     <p><img src="https://static.igem.wiki/teams/4800/wiki/parts/donor.png" width="60%" height="60%"></p>
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     <p><center><img src="https://static.igem.wiki/teams/4800/wiki/parts/donor.png" width="60%" height="60%"></center></p>
 
     <div align="center">
 
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         <strong>Fig1.ackA-pta Donor</strong>
 
         <strong>Fig1.ackA-pta Donor</strong>
 
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     <p>The genes at either end of the acka-pta gene were selected as the upper and lower homology arms and joined by overlap, the upper and lower homology arms were amplified separately, purified and recovered to obtain the acka-pta Donor.  
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     <p>The genes at both end of the ackA-pta gene were selected as the upper and downer homology arms, which were amplified separately, purified and recovered, then joined by overlap to obtain the ackA-pta Donor.
 
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Latest revision as of 05:25, 11 October 2023


ackA-pta Donor

AckA-pta Donor is exogenous DNA used by cells as a template for homologous recombination repair (HDR) after SgRNA and Cas9 complexes have caused DNA double-strand breaks at the target site.


Document

Build:

Fig1.ackA-pta Donor

The genes at both end of the ackA-pta gene were selected as the upper and downer homology arms, which were amplified separately, purified and recovered, then joined by overlap to obtain the ackA-pta Donor.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 969
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 969
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 969
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 969
  • 1000
    COMPATIBLE WITH RFC[1000]