Difference between revisions of "Part:BBa K4585016"
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− | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig 1. The agarose gel | + | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig 1. The agarose gel electrophoresis of pcDNA3.1(+)-GnRH-C linearized vector</p> |
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Latest revision as of 03:06, 11 October 2023
pcDNA3.1(+)-GnRH-C linearized vector
We based on the sequence of the pcDNA3.1(+)-GnRH-C plasmid. Special primers were designed to cut the plasmid into a linear sequence by PCR. The two ends of this linear sequence can be complementary to the GnRH-C homologous recombination fragment to obtain the target product, the pcDNA3.1(+)-GnRH-C plasmid (BBa_K4585011).
1 Diagrams
Fig 1. The agarose gel electrophoresis of pcDNA3.1(+)-GnRH-C linearized vector
2 Caution
The two ends of the pcDNA3.1(+)-GnRH-C linearized vector need to be complementary to the two ends of the corresponding recombinant fragments to ensure the success of homologous recombination ligation.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 249
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 895
Illegal SpeI site found at 249 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 12
Illegal XhoI site found at 901 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 249
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 249
Illegal NgoMIV site found at 1341 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 882