Difference between revisions of "Part:BBa K4895052"
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| − | DeHa2 (Delftia Acidovorans) | + | ===DeHa2 (Delftia Acidovorans)=== |
Haloacid Dehalogenase originating from Delftia Acidovorans, belonging to the α/β hydrolase superfamily. Likely candidate for performing PFAS degradation due to Delftia acidorovans’s ability to subsist solely off of PFOA as a carbon source (Harris, et. al 2022)(10.1016/j.synbio.2022.02.005). | Haloacid Dehalogenase originating from Delftia Acidovorans, belonging to the α/β hydrolase superfamily. Likely candidate for performing PFAS degradation due to Delftia acidorovans’s ability to subsist solely off of PFOA as a carbon source (Harris, et. al 2022)(10.1016/j.synbio.2022.02.005). | ||
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The GC level was optimized to be Medium (0.33 to 0.66). | The GC level was optimized to be Medium (0.33 to 0.66). | ||
Existing cutsites were removed and no new ones were created for the following enzymes: BbsI. | Existing cutsites were removed and no new ones were created for the following enzymes: BbsI. | ||
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| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K4895052 SequenceAndFeatures</partinfo> | ||
Latest revision as of 00:12, 11 October 2023
DeHa2 (Delftia Acidovorans)
Haloacid Dehalogenase originating from Delftia Acidovorans, belonging to the α/β hydrolase superfamily. Likely candidate for performing PFAS degradation due to Delftia acidorovans’s ability to subsist solely off of PFOA as a carbon source (Harris, et. al 2022)(10.1016/j.synbio.2022.02.005).
The sequence was optimized for the organism E.coli (K12). The GC level was optimized to be Medium (0.33 to 0.66). Existing cutsites were removed and no new ones were created for the following enzymes: BbsI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]